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Invitrogen™ Novex™ IEF Anode Buffer (50X)

Catalog No. LC5300
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LC5300 100 mL
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Catalog No. LC5300 Supplier Invitrogen™ Supplier No. LC5300
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Description

An optimized pre-mixed IEF anode buffer

Novex IEF Anode Buffer (50X) is optimized pre-mixed IEF anode buffer for using with Novex IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Isoelectric Focusing, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage Store at room temperature.
Buffer Anode & Cathode Buffers
Concentration 50 X
Gel Type IEF
Gel Compatibility Novex™ IEF Gels
Quantity 100 mL
Shipping Condition Room Temperature
Product Line Novex
Product Type IEF Anode Buffer

Frequently Asked Questions (FAQs)

When staining IEF gels with the Colloidal Blue Staining Kit, is it necessary to use the fixing solution?

Yes, the fix serves two purposes: it fixes the sample in the IEF gel and it helps to remove gel background.

If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.

High background is caused by ampholytes remaining in the gel.
What are the components of the ZOOM IEF Fractionator?

The ZOOM IEF Fractionator includes the following components:

Chamber Assembly Tube with Anode Reservoir
Spill Trough with Cathode Reservoir Lid
Sample Chambers (7)
Sample Chamber O-ring Seals, red (10)
Sample Chamber Caps with O-rings (7)
Cathode End Sealer
Anode End Sealer
Cathode End Screw Cap
Spacers, black (8)
Spares Box 1
Sample Chamber O-ring Seals (8)
Sample Chamber Caps with O-rings (7)
Spares Box 2
Cathode Chamber Seals (2)
Spacers, black (8)

What are the storage conditions for the ZOOM Disks, ZOOM Carrier Ampholytes, ZOOM Focusing buffers, ZOOM Cathode buffer, IEF Anode buffer, and IEF Cathode buffer?

We recommend storing the ZOOM Disks, ZOOM Carrier Ampholytes, ZOOM Focusing buffers, ZOOM Cathode buffer, IEF Anode buffer, and IEF Cathode buffer at 4 degrees C.

For Research Use Only. Not for use in diagnostic procedures.