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Novex™ DYNAL™ Dynabeads™ Protein A and Protein G Immunoprecipitation Kit DFS Item


Provides faster and easier solution for immunoprecipitation experiments than sepharose or agarose

Manufacturer: life technologies  10006D

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Catalog No. 10006D

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Description & Specifications

Specifications

Concentration 30mg/mL
Cross Reactivity Protein A
For Use With (Application) High Throughput-Compatible
Format Bead
Name Protein A Immunoprecipitation Kit
Quantity 40 reactions

Kit includes all reagents needed to perform small scale immunoprecipitation (IP) using antibody of your choice and is optimal for standard IP, co-IP and ChIP.

  • Reduce your protocol time to <30 minutes
  • Decrease or even eliminate background caused by non-specific binding-easily remove of all supernatant after incubation and washing, with no risk of sample loss
  • Minimize antibody usage and maximize target protein recovery-rapid binding kinetics and high capacity
  • Keep protein complexes intact-gentle magnetic separation and rapid binding kinetics avoid physical stress on your precious proteins
  • Improve reproducibility-consistent high performance of the Dynabeads™
  • Binding Capacity: Approximately 250μg hIgG per mL (30) Dynabeads
  • Protein A can be used with most antibodies
  • Starting Samples:Typically cell lysate, plasma, serum, tissue culture, hybridoma supernatants or similar

  • Increase confidence in results-optimized and reproducible buffer compositions
  • Fast and gentle, with no centrifugation, rapid binding kinetics and short protocol time
  • Target proteins experience minimal physical stress, permiting isolation and concentration of labile complexes that might otherwise dissociate or be damaged by proteases during long incubation times
  • Native protein conformation and intact, large protein complexes are preserved
  • Superior signal-to-noise properties
  • Highly reproducible results
  • No columns, centrifugations, or time-consuming pre-treatment of samples
  • Gentle, minimal physical stress on precious proteins
  • Easy handling and simple, short protocol
  • Amount of antibody captured is dependent on concentration in starting sample and type and antibody source

Dynabeads™ Protein A and Dynabeads™ Protein G are also available separately separately (without buffers), both as research products for end-users and in larger volumes for OEM supply.

Immunoprecipitation, Organelle Isolation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis