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Invitrogen™ Novex™ AcTEV™ Protease DFS Item

Specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), ideal for removing affinity tags from fusion proteins

$308.70 - $2,504.25

Specifications

Product Type Protease
Includes AcTEV™ Protease is supplied with a vial of 20X TEV buffer [1M Tris-HCl (pH 8.0), 10mM EDTA] and a vial of 100mM DTT.
Storage Requirements Store at -20°C. Guaranteed stable for 6 months when properly stored.
View More Specs
Includes:  AcTEV™ Protease is supplied with a vial of 20X TEV buffer (1M Tris-HCl (pH 8.0), 10mM EDTA) and a vial of 100mM DTT.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

12575015

DFS Item
 
life technologies
12575015
1000 units Each for $308.70

12575023

DFS Item
 
life technologies
12575023
10,000 units Each for $2,504.25
Description & Specifications

Specifications

Product Type Protease
Includes AcTEV™ Protease is supplied with a vial of 20X TEV buffer [1M Tris-HCl (pH 8.0), 10mM EDTA] and a vial of 100mM DTT.
Storage Requirements Store at -20°C. Guaranteed stable for 6 months when properly stored.

An improved version of Tobacco Etch Virus (TEV) AcTEV™ Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity.

  • Highly specific cleavage activity
  • Enhanced enzyme stability for prolonged protease activity
  • Activity over a broad temperature (4°C to 30°C) and pH (6.0 to 8.5) range
  • Six-histidine sequence to facilitate its removal from the digested protein sample
  • Greater than 85% single-band purity with no nonspecific protease contamination

  • Application
    • Incubation with AcTEV™ Protease releases the protein of interest from the fusion tag
    • This is an effective way to remove solubility, secretion, detection, and purification tags from recombinant protein
    Enzyme specifications
    • Purified from E. coli expressing the AcTEV™ Protease gene
  • Unit definition
    • One unit of AcTEV™ Protease cleaves 85% of a 3μg control substrate in 1 hr at 30°C
  • Unit reaction conditions
    • 50mM Tris-HCl (pH 8.0), 0.5mM EDTA, 1mM DTT, 3μg control substrate, and 1 unit enzyme in 30μL for 1 hr at 30°C AcTEV™ Protease is functionally tested for the absence of any nonspecific protease activity

    GST-Tagged Protein Purification, His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

    Shipping Condition: Dry Ice