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Invitrogen™ NovaFluor™ Antibody Conjugation Kits 
Get the most out of your panel design by conjugating antibodies to NovaFluor dyes specifically designed for spectral hygiene to have narrow emission spectra and minimal cross-laser excitation.
Supplier: Invitrogen™ K06T04L009
Description
NovaFluor Conjugation Kits provide a simple workflow to conjugate a blue, yellow-green or red excitable fluorophore to your antibody of choice in just 3 easy steps. The fluorescence-by-design Phiton platform enables the addition of parameters to your current panels in discovery research and resolves stained populations with near-zero cross excitation, with a lack of emission in the violet channels that plague other fluorophores.
NovaFluor Conjugation Kit provides features including:
- A clean and bright antibody label to increase the number of parameters on conventional and spectral flow cytometers
- Resolve populations with limited/near-zero cross excitation and the lowest spread performance of any blue, yellow-green or red excited fluorescent labels
- Simple 3-step protocol, with 60 sec. of hands-on time for conjugating 10–100 μg of antibody per label
- Up to 70% less excitation when compared to PE tandems, resulting in decreased spillover and higher resolution
3-step conjugation method
- Attach NovaFluor Linker to antibody of choice
- Remove excess NovaFluor Linker from DNA-conjugate antibody
- Anneal NovaFluor to oligo-conjugated antibody
Specifications
| Flow Cytometry | |
| Conjugation Kit | |
| NovaFluor™ |
| 100 μg | |
| NovaFluor™ Blue 555 | |
| 555 nm |
For Research Use Only. Not for use in diagnostic procedures.