missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Culture Media, Supplements, and Reagents
  4. Cell Culture Reagents, Antibiotics and Supplements
  5. Additional Cell Culture Reagents and Supplements
  6. Gibco™ N-2 Supplement (100X)

Gibco™ N-2 Supplement (100X)

Catalog No. p-7076112
Encompass
Change view
Click to view available options
Quantity:
5 mL
50 mL
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
17502048 5 mL
17502001 50 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. 17502048 Supplier Gibco™ Supplier No. 17502048
Only null left

Description

N-2 Supplement is a versatile serum-free cell culture supplement that supports the growth and viability of various cell types.

Gibco N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein's N-1 formulation, containing optimized concentrations of insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite. N-2 Supplement is recommended for growth and expression of neuroblastomas and neural cells in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS).

N-2 Supplement has been demonstrated in wide-ranging applications, such as the culture of neural stem cells, the culture of the SH-SY5Y cell line (subline of the neuroblastoma cell line SK-N-SH), and culture of the HT22 mouse hippocampal neuronal cell line. Additionally, N-2 Supplement adds support to cell culture media used to differentiate pluripotent stem cells (PSCs) to a variety of cell types, including neural and pancreatic-like cell types.

N-2 Supplement may be used with Neurobasal media supplemented with growth factors such as bFGF and EGF. When combined with Dulbecco's Modified Eagle Medium (D-MEM) and fetal bovine serum (FBS), N-2 Supplement also provides suitable supplementation for the culture of human and rodent astrocytes.

  • Each lot is cGMP manufactured
  • Each lot is tested for sterility, endotoxin, and performance
  • Contains insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite
  • Can be used as a substitute for Bottenstein's N-1 formulation
  • Provided as a 100X concentrate

Choose Gibco N-2 Supplement for a reliable, high-quality product trusted by researchers worldwide.

Specifications

Content And Storage Store in freezer (-5 to -20°C) and protect from light.
Cell Type Neural Cells
Classification Serum-free
Concentration 100 X
Culture Type Mammalian Cell Culture
Form Liquid
Product Type Cell Culture Supplement
Serum Level Serum-Free
Sterility Sterile
pH 6 to 8
Quantity 5 mL
Shipping Condition Dry Ice
Tested For Endotoxin, Performance, Sterility
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.