Recombinant Protein;1MG;Mouse CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDD-M52W2) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1 (CD3E) & P04235-1 (CD3D)).;Mouse CD3E&CD3D Heterodimer Protein, Fc, His Tag&Fc, Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.5 kDa (CD3E) and 40.7 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D is fused with a flag tag at the C-terminus. The protein migrates as 55-65 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE). ;Immobilized Biotinylated CD3e Monoclonal Antibody at 1 g/mL (100 L/well) on streptavidin (Cat. No. STN-N5116) (0.5 g/well) plate, can bind Mouse CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-M52W2) with a linear range of 0.016-0.25 g/mL (QC tested).
