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Invitrogen™ Molecular Probes™ SBFI, AM, Cell Permeant DFS Item

Sodium-sensitive molecule used to estimate Na+ gradients in isolated mitochondria, to measure intracellular NA+ levels, or to measure Na+ efflux in cells

Manufacturer:  Invitrogen™ LSS1263

Catalog No. S1263



Description

Description

  • In combination with other fluorescent indicators used to correlate changes in intracellular Na+ with Ca2+ and Mg2+ concentrations, intracellular pH, and membrane potential
  • Although the selectivity of SBFI for Na+ is less than that of calcium indicators such as fura-2, it is sufficient for the detection of physiological concentrations of Na+ in presence of other monovalent cations
  • Label (Ex/Em): SBFI (approximately 340,380/500nm)
  • Lyophilized product may be dissolved in DMSO for use
  • Product is typically loaded into cells by adding the dissolved indicator to medium containing cells
Selectivity Considerations and Cell Loading Strategies
  • Dissociation constant (Kd) of SBFI for Na+ is 3.8mm in absence of K+, and 11.3mm in solutions with a combined Na+ and K+ concentration of 135mm (which approximates physiological ionic strength)
  • SBFI is approximately 18-fold more selective for Na+ than for K+

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Sodium and Potassium Detection

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

Fluorescence Microscope
Store in freezer (-5° to -30°C) and protect from light.
Documents

For Research Use Only. Not for use in diagnostic procedures.