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Invitrogen™ SBFI, AM, cell permeant

Catalog No. S1263
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S1263 1 mg
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Catalog No. S1263 Supplier Invitrogen™ Supplier No. S1263
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Description

Sodium-sensitive molecule used to estimate Na+ gradients in isolated mitochondria, to measure intracellular NA+ levels, or to measure Na+ efflux in cells

  • In combination with other fluorescent indicators used to correlate changes in intracellular Na+ with Ca2+ and Mg2+ concentrations, intracellular pH, and membrane potential
  • Although the selectivity of SBFI for Na+ is less than that of calcium indicators such as fura-2, it is sufficient for the detection of physiological concentrations of Na+ in presence of other monovalent cations
  • Label (Ex/Em): SBFI (approximately 340,380/500nm)
  • Lyophilized product may be dissolved in DMSO for use
  • Product is typically loaded into cells by adding the dissolved indicator to medium containing cells
Selectivity Considerations and Cell Loading Strategies
  • Dissociation constant (Kd) of SBFI for Na+ is 3.8mm in absence of K+, and 11.3 mm in solutions with a combined Na+ and K+ concentration of 135 mm (which approximates physiological ionic strength)
  • SBFI is approximately 18-fold more selective for Na+ than for K+

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Sodium and Potassium Detection

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Cell Viability and Proliferation
For Use With (Equipment) Fluorescence Microscope
Product Type SBFI AM
Dye Type Sodium Indicator
Quantity 1 mg
Shipping Condition Room Temperature

For Research Use Only. Not for use in diagnostic procedures.