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Invitrogen™ LIVE BacLight™ Bacterial Gram Stain Kit, for microscopy & quantitative assays

Catalog No. L7005
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Catalog No. L7005 Supplier Invitrogen™ Supplier No. L7005
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Description

One step assay

The LIVE BacLight™ Bacterial Gram Stain Kit provides a novel one-step fluorescence assay for the determination of gram sign in living bacteria. When gram negative and gram-positive bacterial are simultaneously stained with the membrane-permeant SYTO 9 dye and hexidium iodide, gram-negative bacteria fluoresce green and gram-positive bacteria fluoresce red.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

Order Info

Shipping Condition: Room temperature

Specifications

Color Green, Red
Content And Storage Store in freezer at -5°C to -30°C and protect from light.
Description LIVE BacLight™ Bacterial Gram Stain Kit, for microscopy and quantitative assays
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope, Fluorometer, Flow Cytometer, Microplate Reader
Product Type Bacterial Stain Kit
Dye Type Other Label(s) or Dye(s)
Emission 480 to 700
Excitation Wavelength Range 470 nm
Format Tube(s), 96-well plate, Slide(s)
Product Line BacLight
Quantity 1 kit
Shipping Condition Room Temperature
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Frequently Asked Questions (FAQs)

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).
What is the excitation/emission maxima for SYTO 9 stain and hexidium iodide?

The excitation/emission maxima for these dyes are 480/500 nm for SYTO 9 stain and 480/625 nm for hexidium iodide.

For Research Use Only. Not for use in diagnostic procedures.