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Invitrogen™ HCS Mitochondrial Health Kit

Catalog No. H10295
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H10295 1 kit
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Catalog No. H10295 Supplier Invitrogen™ Supplier No. H10295
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Description

Includes

Kit contains sufficient reagents for performing two plates in a 96-well plate format.

For mitotoxicity and cytotoxicity studies

The HCS Mitochondrial Health Kit simultaneously and quantitatively measures two important cell-health parameters: mitotoxicity and cytotoxicity. The MitoHealth stain detects changes in the mitochondrial membrane potential, whereupon the reagent accumulates in active mitochondria. The kit includes the Image-iT™ DEAD™ Green to detect cytotoxicity, an impermeant dye to healthy cells that becomes permeant when the plasma membrane of cells is compromised. Both indicators are amenable to fixation and permeabilization, thus allowing for multiplexing with other biomarkers of interest. The blue-fluorescent nuclear segmentation tool Hoechst 33342 is also included and stains DNA in both live and dead cells.

Cell Analysis, Cell Structure, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Mitochondria

Order Info

Shipping Condition: Room temperature

Specifications

Color Orange, Blue, Green
Content And Storage The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
  • Store at ≤-20°C, desiccate and protect from light.
    		•
    Detection Method Fluorescence
    For Use With (Equipment) Fluorescence Microscope, High Content Analysis Instrument
    Product Type Mitochondrial Health Kit
    Dye Type Image-iT™ DEAD Green viability stain, MitoHealth stain, Hoechst 33342
    Format 96-well plate
    Product Line Molecular Probes
    Quantity 1 kit
    Shipping Condition Room Temperature

    Frequently Asked Questions (FAQs)

    What is the fluorescence excitation/emission maxima of the stains in the HCS mitochondrial health kit?

    The fluorescence excitation/emission maxima are as follows: Hoechst 33342 - 350/461 nm bound to DNA; Image-iT DEAD Green viability stain - 488/515 nm; MitoHealth stain - 550/580 nm.

    For Research Use Only. Not for use in diagnostic procedures.