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Invitrogen™ Fura Red™, AM, cell permeant

Catalog No. F3021
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10 x 50 μg
500 μg
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Catalog No. Quantity
F3021 10 x 50 μg
F3020 500 μg
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Catalog No. F3021 Supplier Invitrogen™ Supplier No. F3021

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Visible light-excitable fura-2 analog that offers particular possibilities for ratiometric measurement of Ca2+ in single cells

Fura Red, AM is a visible light—excitable fura-2 analog that offers unique possibilities for ratiometric measurement of Ca2+ in single cells by microphotometry, imaging or flow cytometry when used with single excitation, green-fluorescent calcium indicators. This acetoxymethyl (AM) ester form is useful for noninvasive intracellular loading and is also available in 1 mg amounts (F-3020).

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Calcium Indicator
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Fura Red AM
Dye Type Fura Red
Product Line Fura Red
Quantity 10 x 50 μg
Shipping Condition Room Temperature
Excitation Wavelength 488 nm
Emission Wavelength 598 nm
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Frequently Asked Questions (FAQs)

When using Calcium Crimson, AM, as an indicator of intracellular calcium flux, I am not getting a good degree of change. The cells also have GFP. What can I do?

This is a known drawback of Calcium Crimson, AM (as well as Calcium Orange, AM and Fura Red, AM, which are also in the same emission range). You can try increasing the concentration and washing out of any unlabeled dye from the media, to try to get better signal-to-background. If that fails, we recommend using Rhod-3 AM instead, which has a much better change in signal in that wavelength.
Where can I find the manual for the Fura Red, AM, cell permeant (Cat. No. F3020, F3021)?

Unfortunately, we do not have a user manual for the Fura Red, AM, cell permeant (Cat. No. F3021). You can reference section 19.3 of the Molecular Probes handbook for recommendations for use.
There is also a large number of citations & references provided on the product page.

Basically, you can dissolve the product in good quality anhydrous DMSO to prepare a stock solution of 1-10 mM and use a final solution of about 1-10 µM in a physiological buffer. You can then incubate for 15 to 60 minutes, wash and then leave the cells for about 30 minutes to allow the AM group to hydrolyse before starting the assay. You can add Pluronic F-127 (e.g., Cat. No. P6866) to facilitate uptake. Store the DMSO stock solution at -20 degrees C.

For Research Use Only. Not for use in diagnostic procedures.