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Invitrogen™ FocalCheck™ Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration

Catalog No. F36909
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Catalog No. F36909 Supplier Invitrogen™ Supplier No. F36909
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For confocal and fluorescence microscope calibration

The FocalCheck fluorescence microscope test slide #1 is specifically designed for calibrating fluorescence microscope systems and evaluating system and filter performance. The slide contains 10 sample areas coated with FocalCheck ring stained microspheres ranging in size from 0.5-15μm that provide a visual representation of instrument misalignment or other aberrations in row A; row B contains a series of microspheres with fluorescence intensities ranging from 0.667-100%.

Cell Analysis, Cell Cycle, Cell Viability, Proliferation and Function, Cellular Imaging, Immunofluorescence (IF)

Order Info

Shipping Condition: Room temperature

Specifications

Calibration Type Confocal Microscope Calibration, Fluorescence Microscope Calibration
Content And Storage Store at room temperature.
Color Assorted Colors
Product Type Fluorescence Microscope Test Slide
Format Suspension Beads
Product Line FocalCheck
Quantity 1 Ea.
Shipping Condition Room Temperature
What are the excitation/emission wavelengths for the beads in FocalCheck Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration (Cat. No. F36909)?

The spectral characteristics are as follows:

  • A1: 505/515, 560/580, 660/680
  • A2: 365/430 (core), 505/515 (ring)
  • A3-5: 365/430, 505/515, 580/600, 660/680
  • B1-5: 505/515

What type of coverslips are used for the FocalCheck Fluorescence Microscope Test Slide #1 (Cat. No. F36909)?

The coverslips are made of borosilicate glass (#1.5; ~0.17 mm thickness).

Are the microspheres from FocalCheck Fluorescence Microscope Test Slide #1 (Cat. No. F36909) in one plane?

No. Most of the 15 µm beads (Position A1 on the slide) would be within the narrowest focal plane, but they are not guaranteed to all be within the same plane.

For Research Use Only. Not for use in diagnostic procedures.