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Invitrogen™ FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide)

Catalog No. T13320
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10 x 100μg
1mg
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Catalog No. Quantity
T13320 10 x 100μg
T3166 1mg
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Catalog No. T13320 Supplier Invitrogen™ Supplier No. T13320

Lipophilic dye

The styryl dye FM 4-64 has been reported to selectively stain yeast vacuolar membranes with red fluorescence (excitation/emission maxima ∽515/640nm). This dye is an important tool for visualizing vacuolar organelle morphology and dynamics, for studying the endocytic pathway and for screening and characterizing yeast endocytosis mutants. FM 4-64 is also available as 1mg in a single vial (T-3166).

Cell Analysis, Cell Tracing & Tracking, Cell Viability, Proliferation & Function, Endocytosis, Exocytosis & Phagocytosis, General Cell Tracing, Microbial Tracking, Neuronal Tracing

Order Info

Shipping Condition: Room Temperature

Specifications

Color Red
Content And Storage Store at room temperature and protect from light.
For Use With (Equipment) Fluorescence Microscope
Quantity 10 x 100μg
Detection Method Fluorescence
Product Line FM
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Dye
Sub Cellular Localization Plasma Membrane
I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

For Research Use Only. Not for use in diagnostic procedures.