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Invitrogen™ EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit
For measuring MPO activities and high-throughput screening for MPO-specific inhibitors
Supplier: Invitrogen™ E33856
Description
Myeloperoxidase (MPO) is unique peroxidase that, in addition to its peroxidation activity, also catalyzes conversion of hydrogen peroxide (H2O2) and chloride (Cl-) to hypochlorous acid (HOCl). The EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit provides assays for determination of both chlorination and peroxidation activities of MPO in solution and in cell lysates.
- For detection of chlorination, kit includes nonfluorescent 3'-(p-aminophenyl) fluorescein (APF), which is selectively cleaved by hypochlorite (-OCl) to yield fluorescein Peroxidation is detected using nonfluorescent AmplexUltraRed reagent (A36006), which is oxidized by H2O2-generated redox intermediates MPO-I and MPO-II to form fluorescent product
- EnzChek Myeloperoxidase Activity Assay Kit can be used to continuously detect these activities at room temperature over broad dynamic range (1.5 to 200ng/mL)
- Kit combines speed (30 minutes), sensitivity and mix-and-read convenience
Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Nitro-Oxidative Stress, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Reactive Oxygen Species
Order Info
Shipping Condition: Wet Ice
Specifications
| Store in refrigerator 2°C to 8°C and protect from light. | |
| Myeloperoxidase (MPO) Activity Assay | |
| EnzChek | |
| Wet Ice | |
| Myeloperoxidase |
| Fluorescence Intensity | |
| EnzChek Assay Kit | |
| 1 Kit | |
| Chemical Substrate |
For Research Use Only. Not for use in diagnostic procedures.