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Invitrogen™ EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit

Catalog No. E33856
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E33856 1 Kit
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Catalog No. E33856 Supplier Invitrogen™ Supplier No. E33856

Description

For measuring MPO activities and high-throughput screening for MPO-specific inhibitors

Myeloperoxidase (MPO) is unique peroxidase that, in addition to its peroxidation activity, also catalyzes conversion of hydrogen peroxide (H2O2) and chloride (Cl-) to hypochlorous acid (HOCl). The EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit provides assays for determination of both chlorination and peroxidation activities of MPO in solution and in cell lysates.

  • For detection of chlorination, kit includes nonfluorescent 3'-(p-aminophenyl) fluorescein (APF), which is selectively cleaved by hypochlorite (-OCl) to yield fluorescein Peroxidation is detected using nonfluorescent AmplexUltraRed reagent (A36006), which is oxidized by H2O2-generated redox intermediates MPO-I and MPO-II to form fluorescent product
  • EnzChek Myeloperoxidase Activity Assay Kit can be used to continuously detect these activities at room temperature over broad dynamic range (1.5 to 200ng/mL)
  • Kit combines speed (30 minutes), sensitivity and mix-and-read convenience

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Nitro-Oxidative Stress, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Reactive Oxygen Species

Order Info

Shipping Condition: Wet Ice

Specifications

Content And Storage Store in refrigerator 2°C to 8°C and protect from light.
Detection Method Fluorescence
For Use With (Application) Myeloperoxidase (MPO) Activity Assay
Product Type EnzChek Assay Kit
Product Line EnzChek
Quantity 1 Kit
Shipping Condition Wet Ice
Substrate Properties Chemical Substrate
Target Enzyme Myeloperoxidase

Frequently Asked Questions (FAQs)

What is the dynamic range of the EnzChek Myeloperoxidase (MPO) Activity Assay Kit (Cat. No. E33856)?

The EnzChek Myeloperoixdase Activity Assay Kit (Cat. No. E33856) can detect myeloperoxidase activity at a broad dynamic range of 1.5 to 200 ng/mL.

What can interfere with the EnzChek Myeloperoxidase (MPO) Activity assay?

MPO is inhibited by azide, diclofenac, methimazole, quercetin, rutin, and salicylhydroxamic acid. Make certain that samples do not include sodium azide. Endogenous catalases can interfere with the assay; catalase activity may be inhibited by using 3-amino-1,2,4-triazole. Detergents, common components in cell lysis buffers, should be avoided; use freeze/thawing and/or mechanical methods to lyse cells.

For Research Use Only. Not for use in diagnostic procedures.