Invitrogen™ BacMam GFP Transduction Control (BacMam 2.0)

The BacMam 2.0 Transduction Control lets you experience the power and convenience of enhanced BacMam technology, with the convenient readout of emerald GFP (emGFP); our Aequorea victoria emGFP is extremely bright and photostable and this non-targeted form is ideal for testing transduction. Check out how well BacMam 2.0 works with GFP in your model before advancing to the more specialized reagents. The BacMam technology is based on an insect virus (baculovirus) to help efficiently deliver and express genes in mammalian cells. The baculovirus has been modified to include an expression cassette for transgene expression in mammalian cells. BacMam 2.0 incorporate elements that help greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry and genetic elements (enhanced CMV promoter and Woodchuck Post-transcriptional Regulatory Element), that boost expression levels. Baculoviruses do not replicate in mammalian cells and thus have an excellent safety profile and lack cytopathic effects on cells. Cell types previously not compatible with the technology (neuronal cells, T-cells), or only poorly transduced (some stem cells, CHO) are now transduced quantitatively in a simple, one step process.

• Efficient: highly efficient (>90%) transduction of a wide range of mammalian cell lines, including primary cells, stem cells and neuronal cells
• Fast and convenient: Simply add the BacMam 2.0 together with cells to your cover slip or microtiter plate, incubate overnight and assay or store frozen, assay-ready cells for later use
• Safe: non-replicating in mammalian cells, lack of observable cytopathic effect and biosafety level (BSL) 1 handling
• Enables Flexiblity: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies and tightly control expression levels by just varying the dose

Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Fluorescent Protein Assays (GFP)