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Invitrogen™ MitoTracker™ Deep Red FM Dye, for flow cytometry

Catalog No. M46753
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5 vials x 20 tests
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M46753 5 vials x 20 tests
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Catalog No. M46753 Supplier Invitrogen™ Supplier No. M46753
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Description

Mitotracker™ dyes for flow cytometry are cell permeant reagents that label mitochondria in live cells. Provided at an optimized concentration with protocols for flow cytometry to allow for more precise analysis with both conventional and spectral flow cytometry.

  • Cell permeant reagents that label mitochondria in live cells
  • Dyes are provided at an optimized concentration with protocols for flow cytometry to allows for more precise analysis with both conventional and spectral flow cytometry
  • Simply incubate cells in the recommended dye concentration for flow cytometry, which passively diffuses across the plasma membrane and accumulates in active mitochondria
  • Bright colors allow for easy detection of mitochondria using flow cytometry without the need for special filters
  • Dyes are consistent, reliable, and stable allowing for the generation of high-quality data
  • Optimized for flow cytometry use with all essential reagents for labeling mitochondria in a single kit
  • Dissolve a vial of dye in anhydrous DMSO to provide enough dye for 20 assays
  • A range of available colors are detected with standard flow cytometry filters

Specifications

Quantity 5 vials x 20 tests
For Use With (Application) Flow Cytometry
Format 5 x 20 tests
Label or Dye Dye
No. of Tests 100 tests
Regulatory Status RUO
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Our facility does not allow flow cytometry of live cells. Can I fix the cells after staining with MitoTracker Deep Red FM dye?

Yes, this is possible because MitoTracker Deep Red FM dye is retained upon fixation. See Table 2 in this manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp07510.pdf).

What is the difference in using MitoTracker dyes for flow cytometry compared to imaging?

Because of the greater sensitivity of a flow cytometer, you typically use a much lower concentration of reagent than what is typically used for imaging applications. The MitoTracker dyes, for flow cytometry have been optimized for ease of use for most flow cytometry applications.

Is there any difference between the newer MitoTracker dyes for flow cytometry products and the original MitoTracker dye products?

The newer MitoTracker dyes “for flow cytometry” and the original MitoTracker dyes differ only in the packaging. The original MitoTracker dye products are shipped as 20 vials with 50 µg per vial. The newer MitoTracker dyes “for flow cytometry” products are shipped as 5 vials with a proprietary amount of dye per vial. The amount of dye per vial is optimized for flow cytometry experiments. Each vial provides sufficient reagent for 20 flow cytometry experiments.