Norgens microRNA (cel-miR-39) Spike-In Kit offers a quantified synthetic RNA (cel-miR-39) for spike-in during RNA extraction and subsequent normalization in RT-qPCR assays. The amount of cel-miR-39 RNA recovered after RNA extraction is directly correlated with the amount of total RNA recovered. After reverse transcription of the sample RNA (with spike-in), the level of cel-miR-39 can be determined by subjecting the cDNA generated to quantitative PCR using fluorescent nucleic acid stains such as SYBR Green. A cel-miR-39 specific primer is included in the kit. The level of expression of any target transcripts in different RNA samples can be normalized to the cel-miR-39 transcript level using standard methods such as Ct relative quantification.cel-miR-39 RNA is compatible with library preparation methods in Small RNA-Seq workflows. cel-miR-39 RNA can be used for normalization and tracking library construction efficiency.
