MES, is often used as a running buffer for resolving very small proteins on bis-tris gels, also used as a biological buffer in plant cell cultures. It is also been used in various protein characterization assays. This sterile solution suitable for cell culture and molecular biology applications. It is also used as a buffering agent in biology and biochemistry.
Soluble in water (0.5 M at 20°C).
Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2 mm filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.
|0.5M buffer soln.|
|MES buffer soln.|
Safety and Handling
GHS H Statement
Causes skin irritation.
Causes serious eye irritation.
GHS P Statement
Wear protective gloves/protective clothing/eye protection/face protection.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Take off contaminated clothing and wash before reuse.
Specific treatment (see label).
If skin irritation occurs: Get medical advice/attention.
If eye irritation persists: Get medical advice/attention.
TSCA : TSCA