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Invitrogen™ MagniSort™ Mouse CD49b Positive Selection Kit

Catalog No. 501125081
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200 tests
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Catalog No. Quantity
501125081 200 tests
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Contains Sodium azide
Catalog No. 501125081 Supplier Invitrogen™ Supplier No. 8802686274
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Contains Sodium azide

Description

Includes

  • MagniSort™ Anti-Mouse CD49b Biotin : 200 tests, 20μL/test
  • MagniSort™ Positive Selection Beads A

Designed for the magnetic separation of CD49b+ cells by positive selection. It has been optimized for the isolation of CD49b+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD49b antibody and streptavidin-coated magnetic beads.

  • CD49b+ cells are bound by antibody and then magnetic beads
  • When placed in a magnetic field, the undesired cells can be separated from CD49b+ cells by decanting
  • After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (integrin alpha 2), clone DX5, and Anti-Mouse NK1.1, clone PK136. For mouse strains that do not express NK1.1 antigen such as BALB/c, SJL, AKR, CBA C3H, anti-Mouse CD335 (NKp46), clone 29A1.4, may be used
  • For higher purity of CD49b+ cells, 10 μL/test of beads may be used, instead of 20 μL as indicated in the protocol, however, this may result in decreased recovery rate of CD49b+ cells

Specifications

Cell Type CD49b+
Product Type Positive Selection Kit
No. of Tests 200 tests
Quantity 200 tests
Target Species Mouse

For Research Use Only. Not for use in diagnostic procedures.