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Thermo Scientific™ MagnaBind™ Protein G Beads
Description
Thermo Scientific™ MagnaBind™ Beads provide a convenient method for magnetic separation of antibodies, antigens, lectins, enzymes, nucleic acids and cells using affinity binding. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.
Features of MagnaBind Protein G Beads:
- Composition: Silanized iron oxide
- Magnetization: 25-35EMU/g
- Type of Magnetization: Superparamagnetic (no magnetic memory)
- Surface Area: >100m2/g
- Bead Size: 1-4μm diameter
- Settling Rate: 4% in 30 minutes
- Effective Density: 2.5g/mL
- Number of Beads: 1 x 108 beads/mg
- pH Stability: Aqueous solution, above pH 4.0
- Concentration: 5 mg/mL
MagnaBind Protein G Beads are typically used to isolate antibodies from serum and cell culture supernatant and to separate cells of interest from a cell mixture. For antibody purification, the beads are incubated with the antibody solution and then magnetically separated from the supernatant. The antigens and antibodies are then dissociated from the beads using an elution buffer. For cell separation, the monoclonal or polyclonal antibody to the cell surface antigen is incubated with Protein G magnetic beads, magnetically separated, and then incubated with the cell suspension.
Applications:
- Cell sorting using either positive or negative selection
- Protein purification or immunoassays using either direct or indirect methods
Specifications
Specifications
| Capacity (Metric) | 5 mL |
| Concentration | Slurry: 5 mg/mL |
| Description | MagnaBind Protein G Beads |
| Content And Storage | Upon receipt store products at 4°C. Do not freeze products. Products are shipped at ambient temperature. |
| Particle Size | 1 to 4 μm |
| Form | Liquid |
| Product Line | MagnaBind |
| Quantity | 5 mL |
| Target | Antibody |
| Type | Magnetic Affinity Bead |
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Frequently Asked Questions (FAQs)
Protein A, Protein G, and Protein A/G bind almost exclusively to the IgG class of antibodies, but their binding properties differ among species and subclasses of IgG. Protein L binds in the variable fragment of some kappa light chains and can react with any immunoglobulin, not just IgG, as long as the correct kappa light chains are present. Protein L does not bind lambda light chains and certain kappa chains of different species.
-Protein A is generally preferred for rabbit, pig, dog, and cat IgG.
-Protein G has better binding capacity for a broader range of mouse and human IgG subclasses (e.g., IgG1 vs. IgG2)
-Protein A/G is a recombinant fusion protein that includes the IgG-binding domains of both Protein A and Protein
G. Therefore, Protein A/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human, and other mammalian samples.
-Protein L binds to certain immunoglobulin kappa light chains. Because kappa light chains occur in members of all classes of immunoglobulin (i.e., IgG, IgM, IgA, IgE and IgD), Protein L can purify these different classes of antibody. However, only those antibodies within each class that possess the appropriate kappa light chains will bind. Generally, empirical testing is required to determine if Protein L is effective for purifying a particular antibody. It binds only Vk1 in mouse and VkI, VkIII and VkIV in human.
Read more about the general characteristics of Ig-binding proteins (https://www.thermofisher.com/us/en/home/life-science/antibodies/antibody-purification-kits-reagents.html) and (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0034-Ab-binding-proteins.pdf).
For Research Use Only. Not for use in diagnostic procedures.