Thermo Scientific™ MagnaBind™ Protein A Beads

Description

Thermo Scientific™ MagnaBind™ Beads provide a convenient method for magnetic separation of antibodies, antigens, lectins, enzymes, nucleic acids and cells using affinity binding. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

Features of MagnaBind Protein A Beads:

• Composition: Silanized iron oxide
• Magnetization: 25-35EMU/g
• Type of Magnetization: Superparamagnetic (no magnetic memory)
• Surface Area: >100m²/g
• Bead Size: 1-4μm diameter
• Settling Rate: 4% in 30 minutes
• Effective Density: 2.5g/mL
• Number of Beads: 1 x 10⁸ beads/mg
• pH Stability: Aqueous solution, above pH 4.0
• Concentration: 5 mg/mL

MagnaBind Protein A Beads are typically used to isolate antibodies from serum and cell culture supernatant and to separate cells of interest from a cell mixture. For antibody purification, the beads are incubated with the antibody solution and then magnetically separated from the supernatant. The antigens and antibodies are then dissociated from the beads using an elution buffer. For cell separation, the monoclonal or polyclonal antibody to the cell surface antigen is incubated with Protein A magnetic beads, magnetically separated, and then incubated with the cell suspension.

Applications:

• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods