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Molecular Probes™ LIVE/DEAD™ Violet Viability/Vitality Kit, for 405 nm excitation

Catalog No. 501121523
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Catalog No. 501121523 Supplier Molecular Probes™ Supplier No. L34958

Description

The LIVE/DEAD™ Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health.

  • Provides a two-color fluorescence cell viability and vitality assay
  • Based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity
  • Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application
  • Both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes

Specifications

Cell Permeability Impermeant
Cell Type Eukaryotic Cells
Color Aqua, Violet
Content And Storage Contains 5 vials of calcein violet stain (25 μg/vial), 5 vials of aqua-fluorescent reactive dye, and 1 vial of DMSO.

Store in freezer (-5°C to -30°C) and protect from light.
Description LIVE/DEAD™ Violet Viability/Vitality Kit, for 405 nm excitation
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Flow Cytometer
Product Type Stain
Dye Type Other Label(s) or Dye(s)
Emission Aqua-fluorescent reactive dye: 367/526, Calcein violet stain: 400/452
Excitation Wavelength Range 367/400 nm
Form Lyophilized
Format Tube(s)
Product Line LIVE/DEAD
Quantity 1 kit
Shipping Condition Room Temperature
Solubility DMSO (Dimethylsulfoxide)
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Frequently Asked Questions (FAQs)

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

For Research Use Only. Not for use in diagnostic procedures.