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Molecular Probes™ LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green 
The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells.
Supplier: Molecular Probes™ L34951
Description
- A simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells
- Assay is based on the reduction of C12-resazurin to red-fluorescent C12-resorufin in metabolically active cells, and on the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells)
- In this assay, dead cells emit mostly green fluorescence and healthy, metabolically active cells emit mostly red fluorescence
- Injured cells exhibit reduced red and green fluorescence
Specifications
| Impermeant, Permeant | |
| Green, Red | |
| LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green | |
| Viability Assay | |
| Cell Vitality Assay Kit | |
| C12 Resazurin: 563/587 SYTOX™ Green: 504/523 |
|
| Tube(s) | |
| 1000 Assays kit | |
| DMSO (Dimethylsulfoxide) |
| Mammalian Cells, Eukaryotic Cells | |
| Contains 5 vials of C12-resazurin (40 μg each vial), 1 vial of SYTOX™ green (100 μL, 10 μM solution in DMSO), 1 vial of DMSO (1.5 mL), and 10X phosphate buffer (100 mL). Store in freezer (-5°C to -30°C) and protect from light. |
|
| Fluorescence | |
| Fluorescence Microscope, Flow Cytometer, Microplate Reader | |
| Other Label(s) or Dye(s) | |
| 504/563 nm | |
| LIVE/DEAD, SYTOX | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.