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Molecular Probes™ LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green

Catalog No. 501121522
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1000 Assays kit
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501121522 1000 Assays kit
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Catalog No. 501121522 Supplier Molecular Probes™ Supplier No. L34951
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Description

The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells.

  • A simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells
  • Assay is based on the reduction of C12-resazurin to red-fluorescent C12-resorufin in metabolically active cells, and on the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells)
  • In this assay, dead cells emit mostly green fluorescence and healthy, metabolically active cells emit mostly red fluorescence
  • Injured cells exhibit reduced red and green fluorescence

Specifications

Cell Permeability Impermeant, Permeant
Cell Type Mammalian Cells, Eukaryotic Cells
Color Green, Red
Content And Storage Contains 5 vials of C12-resazurin (40 μg each vial), 1 vial of SYTOX™ green (100 μL, 10 μM solution in DMSO), 1 vial of DMSO (1.5 mL), and 10X phosphate buffer (100 mL).

Store in freezer (-5°C to -30°C) and protect from light.
Description LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product Type Cell Vitality Assay Kit
Dye Type Other Label(s) or Dye(s)
Emission C12 Resazurin: 563/587
SYTOX™ Green: 504/523
Excitation Wavelength Range 504/563 nm
Format Tube(s)
Product Line LIVE/DEAD, SYTOX
Quantity 1000 Assays kit
Shipping Condition Room Temperature
Solubility DMSO (Dimethylsulfoxide)
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For Research Use Only. Not for use in diagnostic procedures.