Thermo Scientific™ LanthaScreen™ TR-FRET RAR gamma Coactivator Assay Kit, goat

Numéro de catalogue. PV4411

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800 x 20 μL assays

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PV4411	800 x 20 μL assays

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Numéro de catalogue. PV4411 Fournisseur Thermo Scientific™ Code fournisseur PV4411

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Description

Contains Goat Tb-Anti-GST antibody

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15123:
The LanthaScreen™ TR-FRET Retinoic Acid Receptor (RAR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RAR gamma ligands as agonists of coactivator recruitment.
The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
The LanthaScreen™ TR-FRET RAR gamma Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human RAR gamma ligand-binding domain (RAR gamma-LBD) that is tagged with glutathione-S-transferase (GST).
To assay, RAR gamma-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.
The LanthaScreen™ TR-FRET RAR gamma Coactivator Assay Kit contains RAR gamma-LBD (GST) protein, fluorescein-labeled PGC1a coactivator peptide, Tb-anti-GST antibody, and buffers.
Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Conjugate	Tb (Terbium)
Detection Method	Fluorescence
For Use With (Equipment)	Microplate Reader
Quantity	800 x 20 μL assays
Packaging	384-well Plate
Content And Storage	Store in ultra-cold freezer (-68 to -85°C).
Assay Entry	Biochemical coregulator interaction
Gene ID (Entrez)	5916
Readout	End Point
Shipping Condition	Dry Ice
Target Entry	RARG, RAR gamma, NR1B3
Product Line	LanthaScreen
For Use With (Application)	Co-Factor Interaction Assay, TR-FRET
Ligand	RAR gamma
Product Type	TR-FRET RAR Gamma Coactivator Assay Kit
No. of Assays	800 x 20 μL Assays
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For Research Use Only. Not for use in diagnostic procedures.