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Gibco™ Laminin Mouse Protein, Natural

Catalog No. 23017015
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23017015 1 mg
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Catalog No. 23017015 Supplier Gibco™ Supplier No. 23017015

Description

Laminin Mouse Protein, Natural is the major glycoprotein component of basement membranes, and has a molecular weight of 850,000 daltons.

Laminin is the major glycoprotein component of basement membranes, and has a molecular weight of 850,000 daltons. This preparation of mouse Laminin was isolated from the Engelbreth-Holm-Swarm (EHS) sarcoma and is supplied at a concentration of 0.5-2.0 mg/mL.

Key functions include:

  • Modulation of cell attachment, cell spreading, cell growth and motility
  • Binding to itself and other matrix compounds
  • Promotion of epithelial differentiation
  • Modification of leukocyte function
  • Stimulation of neurite outgrowth

Supplied: 1 mg in 50 mM Tris-HCL (pH 7.4), 0.15 M NaCl

Performance qualification (cell attachment): Half-maximal cell attachment of MG63 cells at 37°C: ≤20 μg/mL

Specifications

For Use With (Application) Cell Culture
Quantity 1 mg
Packaging Vial
Gene Alias Laminin
Product Type Mouse laminin
Conjugate Unconjugated
Species Mouse
Recombinant Native
Sterility Sterile
Content And Storage Store in ultra-cold freezer (-68 to -85°C) and protect from light.
Shipping Condition Dry Ice
Form Frozen
Protein Subtype Laminin
Research Category Extracellular Matrix & Cell Adhesion, Stem Cell Research
Color Clear
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Frequently Asked Questions (FAQs)

What is the molecular weight of Laminin Mouse Protein, Natural?

It has 3 subunits and has a MW of 850 kDa: one A chain is 400 kDa and two B chains are 205 and 215 kDa.
I would like to know how Laminin Mouse Protein, Natural was sterilized.

Laminin Mouse Protein, Natural (Cat. No. 23017015) is sterile filtered through a 0.22 micron filter membrane.

Can I use the Poly-D-Lysine matrix with other coatings?

iPSC-derived neurons have been cultured successfully on Poly-D-Lysine with a secondary coating of mouse and human laminin.
Can natural mouse laminin be thawed, aliquoted, and refrozen?

Yes, it is recommended to store usage size aliquots at -68 degrees C to -85 degrees C.
What is the protein concentration per vial for natural mouse laminin?

The protein concentration per vial is 1 mg/vial. The specification for protein concentration can range from 0.5 to 2.0 mg/mL. The volume per vial can vary as it will be based on the protein concentration determined for the specific lot. The volume added to each vial will equal 1 mg of laminin.

For Research Use Only. Not for use in diagnostic procedures.