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Thermo Scientific™ KingFisher™ PlasmidPro Maxi Processor, extended warranty package

Catalog No. A66427
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Catalog No. A66427 Supplier Thermo Scientific™ Supplier No. A66427

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

The KingFisher™ PlasmidPro Maxi Processor transforms the maxi-scale plasmid purification process with a cutting-edge, fully automated workflow that eliminates labor-intensive centrifugation and pipetting steps.

The KingFisher™ PlasmidPro Maxi Processor transforms the maxi-scale plasmid purification process with a cutting-edge, fully automated workflow that eliminates labor-intensive centrifugation and pipetting steps. Simply pour in the culture and let the instrument handle the rest. In under 75 minutes, this innovative system efficiently executes all essential purification steps, delivering high-quality, endotoxin-free plasmid DNA.

Features and Benefits of the KingFisher™ PlasmidPro Maxi Processor include:

True walkaway automation

The KingFisher™ PlasmidPro system is fully automated, eliminating the need for pipetting or centrifugation steps. It completes each run in under 75 minutes, allowing you to focus on other tasks.

Exceptional quality

Optimized for maxi-scale Plasmid DNA purification from 150 mL bacterial cultures, the system consistently delivers high-quality, advanced transfection grade plasmid DNA.

Convenient pre-filled cartridge

The one-piece, pre-filled KingFisher™ PlasmidPro Maxi Processor Endotoxin-Free Cartridge eliminates setup time and ensures a simple clean-up process, enabling quick turnaround for your next purification.

Effortless installation and setup

The installation process can be completed in minutes on any benchtop, ensuring immediate usability.

AB Assurance Plan

The AB Assurance Plan is a comprehensive repair plan that aims to optimize instrument performance and ensure the availability of critical systems. It includes preventive maintenance, proactive instrument monitoring, and repairs, all aimed at keeping your laboratory operating smoothly.

Additional details

The KingFisher™ PlasmidPro Maxi Processor is a cutting-edge solution designed for fully automated maxi-scale plasmid purification. This advanced system is specifically optimized for cultures grown in Luria-Bertani (LB) medium.

With its innovative self-contained cartridge, pre-filled with the necessary reagents, the KingFisher™ PlasmidPro minimizes handling, preparation, and clean-up time.

At the end of the procedure, the system delivers endotoxin-free plasmid DNA resuspended in TE buffer, ensuring optimal quality and compatibility with downstream applications.

Specifications

Sample Type Bacterial Culture
Format Cartridge
Dimensions 19.7 x 11.8 x 23.6 in. (50 x 30 x 60 cm)
Display 7 in. Touchscreen
For Use With (Application) Plasmid purification, advanced transfection applications
Ports USB
Purification Target Plasmid DNA
Quantity 1 each
Run Time <75 min.
Samples per Run 1 sample/run
Sample Volume 150 mL
Shipping Condition Room temperature
Memory ∼30 GB
Electrical Requirements 100-240 VAC, 50/60 Hz (nominal), automatic voltage detection
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Frequently Asked Questions (FAQs)

Can I resuspend a frozen pellet for use with the KingFisher PlasmidPro Maxi Processor?

When using frozen pellets as a starting point, please consider the following guidelines:

  • The pellet should represent 150 mL of the specified culture.
  • Fully resuspend the pellet in 150 mL of 1X PBS or the parent media.
  • The resuspension can be achieved by either pipetting up and down or vigorous vortexing.
What are the storage conditions for the KingFisher PlasmidPro Maxi Processor Cartridge? Is refrigeration required?

We recommend storing the KingFisher PlasmidPro Maxi Processor Cartridge at room temperature. Refrigeration is not needed.
Can I reuse the KingFisher PlasmidPro Maxi Processor Cartridge?

The KingFisher PlasmidPro Maxi Processor Cartridge is single-use and should be disposed of after use, minimizing clean-up and risk of cross-contamination. Re-use of the cartridge will contaminate and potentially harm the instrument.
Is the KingFisher PlasmidPro Maxi Processor Cartridge animal origin-free?

No. The RNaseA used in the KingFisher PlasmidPro Maxi Processor Cartridge is not animal origin-free. Review the Certificate of Analysis (COA) for more details.

What solution is the plasmid DNA sample resuspended in at the end of the purification using the KingFisher PlasmidPro Maxi Processor?

At the end of the purification using the KingFisher PlasmidPro Maxi Processor, the plasmid DNA sample is resuspended in TE buffer (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA).
What run data is generated and/or captured by the KingFisher PlasmidPro Maxi Processor?

The KingFisher PlasmidPro Maxi Processor is designed to automatically capture the following information for every run:

  • Cartridge Lot Number
  • Cartridge Expiration Date
  • Sample Name
  • Sample Run Start Time
  • Sample Run Finish Time
  • Run Errors (Type/Details)

By automatically reading the barcodes, the KingFisher PlasmidPro Maxi Processor helps ensure accurate and efficient tracking of important information related to the cartridge, samples, and run details.
Is there any required downtime between runs on the KingFisher PlasmidPro Maxi Processor?

No. The KingFisher PlasmidPro Maxi Processor does not require any downtime between runs. The system does not employ any mechanical, optical, or electrical components that have a “wait” time to reset between purification runs. As soon as the used cartridge is removed from the instrument, a new cartridge can be inserted and run immediately.
What is the total run-time for the purification process using the KingFisher PlasmidPro Maxi Processor?

The run time for the purification process is 73 min.
What are the guidelines for growing E. coli for optimal yield and purity results when using the KingFisher PlasmidPro Maxi Processor, e.g., OD range, growth media, plasmid size?

  • Use overnight Luria-Bertani medium optimally grown for at least 12 hr but not greater than 16 hr. Grow sufficient culture volume to support 150 mL.
  • Use bacterial cultures with a measured cell density of approximately 1.5 - 6.0 absorbance at 600 nm (A600).
  • Use a high-copy or low-copy plasmid between 3 and 10 kb cultured in Luria-Bertani medium to obtain maximal yield and purity of plasmid DNA.

Note: Cell density or plasmid kb outside of the recommendation will result in reduced yield and purity.
How does the KingFisher PlasmidPro Maxi Processor work?

The KingFisher PlasmidPro Maxi Processor is designed for fully automated, maxi-scale plasmid purification from cultures grown in Luria-Bertani medium or Terrific Broth. The instrument utilizes a self-contained cartridge pre-filled with the necessary reagents to process the culture by filtration and alkaline lysis, to minimize handling and preparation time. It uses a system of pumps and valves to move the sample and reagents in the cartridge to perform all the steps of the purification process in under 75 mins and provides endotoxin-free plasmid DNA resuspended in TE buffer, at the end of the procedure.

For Research Use Only. Not for use in diagnostic procedures.