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Alexa Fluor™ Kinase Tracer 199, 25 μM

Catalog No. PV5830
Encompass
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25 μL
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PV5830 25 μL
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Catalog No. PV5830 Supplier Alexa Fluor™ Supplier No. PV5830
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Description

Alexa Fluor™ Kinase Tracer 199 is detected by addition of a Eu-labeled anti-tag antibody.

  • Color: Far-Red
  • Excitation/Emission (nm): 340/665
  • Label or Dye: Alexa Fluor™ 647
  • Target Enzyme: Kinases (Multiple)
  • Validated Kinases: EGFR T790M L858R, EPHB4, KIT V654A, MAPK9 (JNK2), RIPK2
  • In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 199 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.

Specifications

Assay Kinase Binding Assay
Assay Entry Biochemical binding/kinetic
Color Far-Red
Content And Storage Store at -20°C.
Detection Method Fluorescence
Excitation/Emission 340/665
For Use With (Application) TR-FRET
Label or Dye Alexa Fluor™ 647
Quantity 25 μL
Shipping Condition Dry Ice
Target Entry MAPK8, EGFR, RIPK2, MAPK9, EGFR T790M L858R, MAPK14, MAPK10, EGFR L861Q, EGFR L858R
Type Kinase Tracer
Validated Kinases EGFR T790M L858R, EPHB4, KIT V654A, MAPK9 (JNK2), RIPK2
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For Research Use Only. Not for use in diagnostic procedures.