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Thermo Scientific™ Kinase Tracer 178, 25 μM

Catalog No. PV5593
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Quantity:
25 μL
Catalog No. Quantity
PV5593 25 μL
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Catalog No. PV5593

Supplier: Thermo Scientific™ PV5593

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Description

In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 178 to a kinase is detected by addition of a Eu-labeled anti-tag antibody.

In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 178 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.

Specifications

Product Line LanthaScreen
For Use With (Equipment) Microplate Reader
Target Kinases (Multiple)
For Use With (Application) TR-FRET
Assay Kinase Binding Assay
Content And Storage Store at -20°C.
Assay Entry Biochemical binding/kinetic
Label or Dye Alexa Fluor 647
Product Type Kinase Tracer
Quantity 25 μL
Format 384-well plate
Readout Kinetic
Shipping Condition Dry Ice
Target Entry PTK6, DDR2, BMPR1A, RAF1 (cRAF) Y340D Y341D, ACVR1B, WNK2, TEC, DDR1, EPHB4, ERBB4, KIT V654A, TGFBR1, NLK, TGFBR2, EPHB3, BRAF V599E, BRAF, ACVR2B, ACVR2A
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For Research Use Only. Not for use in diagnostic procedures.