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Invitrogen™ Jump-In™ CHO-K1 Kit

Jump-In™ CHO-K1 Kit

Supplier: Invitrogen™ A14148

Catalog No. A14148

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

The Jump-In™ CHO-K1 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ CHO-K1 cell line, to create an isogenic stable cell line with less effort and in less time than traditional cell engineering methods.

The high retargeting efficiency, made possible by the R4 sites in the CHO-K1 cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for the easy selection of a positive stable clone expressing your gene of interest.

The Jump-In™ CHO-K1 Kit Lets You:

Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods

Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms, or orthologs

Generate multiple cell lines in parallel using the simplified work flow

Easily access the technology without complicated licenses or restrictions to interpret

Save Time with Rapid and Efficient Generation of Engineered Cell Lines
With the Jump-In™ CHO-K1 Kit you can generate functional cell pools in as little as 2 weeks without laborious clone isolation and analysis, and the streamlined protocol makes it easier to generate several cell lines at the same time. Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones. In addition, the Jump-In™ technology gives you the freedom to generate an unlimited number of cell lines without restrictive licensing requirements.

Expand Your Experimental Capabilities
The Jump-In™ CHO-K1 Kit is the ideal solution for cells and assays where transient engineering technologies are problematic, as well as for difficult to The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs. Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway™ destination vectors accept large inserts.

The kit includes:

pJTI™ R4 Dest CMV pA vector (100μg)

pJTI™ R4 Int vector (100μg)

Jump-In™ CHO-K1 Cells (2 vials @ 1 ml each)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Greener Choice Claims

Environmental benefits include:

Renewable Energy

Specifications

Product Line	Jump-In™
Product Type	CHO-K1 Kit
Cell Line	Jump-In™ CHO-K1
Expression System	Mammalian
Promoter	CMV
System Type	Jump-In™

Content And Storage	The kit includes: Jump-In™ CHO-K1 cells pJTI™ R4 Int (integrase vector) pJTI™ R4 DEST CMV pA (destination vector) Upon receipt, immediately store the cells in liquid nitrogen and the vectors at -20 °C. Cells stored at -80°C can quickly lose viability.
Quantity	1 kit
Format	Kit
Key Functions	Stable Cell Line Development, Targeted Integration
Selection Agent (Eukaryotic)	Hygromycin

Product Suggestions Documents

Documents

Product Information

Manuals

Jump-In Parental Cell Line Kit Application Note File size: 1.8 MB

Product Sheet: Jump-In CHO-K1 Retargeting Kit Version: MAN0005285 Rev A.0 (20 June 2017) File size: 132.3 kB

Jump-In CHO-K1 Retargeting Kit User Guide Version: MAN0005450 Rev A.0 (19 June 2017) File size: 1.7 MB

For Research Use Only. Not for use in diagnostic procedures.