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Invitrogen™ Yeast Nitrogen Base

Catalog No. Q30009
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Q30009 500 g
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Catalog No. Q30009 Supplier Invitrogen™ Supplier No. Q30009
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Description

Includes

Each pouch contains reagents to prepare 500mL of 10X YNB for Pichia media or 1000mL of 10X YNB for S. cerevisiae media.

Base medium for preparation of minimal and synthetic defined yeast media

  • YNB contains ammonium sulfate but does not contain amino acids
  • Autoclavable

Cloning, Transformation

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage Each bottle contains 500 g of reagent. Use 67 g to prepare 500 mL of 10X YNB for Pichia media or 1,000 mL of 10X YNB for S. cerevisiae media.

Store at room temperature. Guaranteed stable for 12 months when properly stored.
Form Powder
Media Type YNB
Preparation Method Autoclave
Product Type Microbiology Media
Target Organism Class P. pastoris, S. cerevisiae
Final Product Type Liquid Medium
Material Powder Reagents
Packaging Type 500 g/Bottle
Quantity 500 g
Shipping Condition Room Temperature
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Frequently Asked Questions (FAQs)

What are the components and concentrations for 1X YNB (yeast nitrogen base)?

For the pouches sold as Cat. No. Q30007, each pouch contains reagents to prepare 500 mL of 10X YNB for Pichia media or 1,000 mL of 10X YNB for S. cerevisiae media (since Pichia media is 2X what is used for S. cerevisiae). Below is the formulation for 1X YNB:

Ammonium sulfate, 5 g
Biotin, 0.002 mg
Calcium pantothenate, 0.4 mg
Folic acid, 0.002 mg
Inositol, 2 mg
Niacin, 0.4 mg
Para-aminobenzoic acid, 0.2 mg
Pyridoxine HCl, 0.4 mg
Riboflavin, 0.2 mg
Thiamine HCl, 0.4 mg
Boric acid, 0.5 mg
Copper sulfate, 0.4 mg
Potassium iodide, 0.1 mg
Ferric chloride.6H2O, 0.2 mg
Manganese sulfate.H2O, 0.4 mg
Sodium molybdate.2H2O, 0.2 mg
Zinc sulfate.H2O, 0.4 mg
Potassium phosphate monobasic, 1 g
Magnesium sulfate, 0.5 g
Sodium chloride, 0.1 g
Calcium chloride, 0.1 mg
Can YNB medium (with ammonium sulfate, without amino acids) be autoclaved instead of filter-sterilized?

Yes, YNB medium with ammonium sulfate and without amino acids can be autoclaved for sterilization.

For Research Use Only. Not for use in diagnostic procedures.