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Invitrogen™ Terminal Deoxynucleotidyl Transferase, Recombinant DFS Item

Performance and quality tested

$226.80 - $579.60

Specifications

Storage Requirements Store at -20°C
View More Specs
Includes:  Vial of 5X buffer [500mM potassium cacodylate (pH 7.2), 10mM CoCl2 , 1mM DTT]

 Alert

Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical. See MSDS.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

10533065

DFS Item
 
life technologies
10533065
500 units Each for $226.80

10533073

DFS Item
 
life technologies
10533073
3 x 500 units Each for $579.60
Description & Specifications

Specifications

Storage Requirements Store at -20°C

Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is DNA polymerase that catalyzes addition of deoxynucleotides to 3fi hydroxyl terminus of DNA.

  • Source: Purified from baculovirus clone of calf thymus TdT
  • Performance and Quality Testing: Endonuclease, 3' and 5' exodeoxyribonuclease, and levels of incorporation tested
  • Unit Definition: One unit incorporates 1nmol dATP into acid-precipitable material in 1 hr. at 37°C, using d(pA)50 as primer
  • Unit Reaction Conditions: 0.2M potassium cacodylate (pH 7.2), 10mM MgO4 C4 H6 , 1mM 2-mercaptoethanol, 0.5mg/mL BSA, 100 flM d(pA)50, 1mM [3H]dATP, and enzyme in 0.15mL for 1 h at 37°C

Cloning; Restriction Enzyme Cloning; Homopolymer tailing of vector and insert for cloning; Labeling oligonucleotides with biotin, 32P- or 35S-label; Apoptosis (TUNEL)

Shipping Conditions: Approved for shipment on wet or dry ice