Invitrogen™ SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays
Conveniently contains all of the reagents required for double-stranded cDNA synthesis within the GeneChip™ workflow
Manufacturer: Invitrogen™ A10752030
The kit includes Invitrogen's proprietary RNase H reduced activity SuperScript™ II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix– protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).
Shipping Condition: Dry Ice
|Poly(A+) RNA, Total RNA|
|For generating double-stranded cDNA|
|50 µM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript™ II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water
All components are guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.