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Invitrogen™ SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays

Catalog No. A10752030
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30 Reactions
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A10752030 30 Reactions
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Catalog No. A10752030 Supplier Invitrogen™ Supplier No. A10752030
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Description

Includes

50μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10mM dNTP, SuperScript™ II (200u/μL), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water

Conveniently contains all of the reagents required for double-stranded cDNA synthesis within the GeneChip™ workflow

The kit includes Invitrogen's proprietary RNase H reduced activity SuperScript™ II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix– protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).

Order Info

Shipping Condition: Dry Ice

Specifications

Content And Storage 50 μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript™ II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water
All components are guaranteed stable for 6 months when properly stored.
Detection Method Biotin-based
Format Kit
Label or Dye Biotin
Final Product Type cDNA (Double-stranded)
Sample Type Poly(A+) RNA, Total RNA
Labeling Target cDNA
For Use With (Application) For generating double-stranded cDNA
Includes Label or Dye No
For Use With (Equipment) Affymetrix GeneChip™
Labeling Method Direct Labeling
Product Line SuperScript
Product Type cDNA Kit
Quantity 30 Reactions
Shipping Condition Dry Ice
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For Research Use Only. Not for use in diagnostic procedures.