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Invitrogen™ Ribonuclease H DFS Item

Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA

$203.70 - $722.40

Specifications

Storage Requirements Store at -20°C in a non-frost-free freezer
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Includes:  Ribonuclease H containing 30 units supplied at a concentration of 2U/μL

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

18021014

DFS Item
 
life technologies
18021014
30 units Each for $203.70

18021071

DFS Item
 
life technologies
18021071
120 units Each for $722.40
Description & Specifications

Specifications

Storage Requirements Store at -20°C in a non-frost-free freezer

  • Source: Purified from E. coli expressing the E. coli RNase H gene on a plasmid
  • Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
  • Unit definition: One unit hydrolyzes 1nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
  • Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl2 , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol 3H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min. at 37°C

Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription

Shipping Conditions: Approved for shipment on wet or dry ice