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Invitrogen™ Ribonuclease H 
Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA
$290.00 - $960.00
Specifications
| Concentration | 2 U/μL |
|---|---|
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
| Enzyme | RNase |
| Product Type | Ribonuclease H |
Includes: Ribonuclease H containing 30 units supplied at a concentration of 2U/μL
Description
- Source: Purified from E. coli expressing the E. coli RNase H gene on a plasmid
- Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
- Unit definition: One unit hydrolyzes 1nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
- Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl2 , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol 3H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min. at 37°C
Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription
Order Info
Shipping Conditions: Approved for shipment on wet or dry ice
Specifications
| 2 U/μL | |
| Approved for shipment on Wet or Dry Ice | |
| RNase | |
| Ribonuclease H |
For Research Use Only. Not for use in diagnostic procedures.