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Invitrogen™ M-MLV Reverse Transcriptase Buffer

Catalog No. 18057018
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18057018 1 mL
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Catalog No. 18057018 Supplier Invitrogen™ Supplier No. 18057018
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Description

Includes

1mL M-MLV Reverse Transcriptase Buffer and 500μL DTT (0.1M)

M-MLV Reverse Transcriptase Buffer is for use with Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT).

M-MLV Reverse Transcriptase Buffer is for use with Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT).

5X buffer composition
• 250 mM Tris-HCl (pH 8.3)
• 375 mM KCl
• 15 mM MgCl2

Included with
• M-MLV Reverse Transcriptase
• SuperScript Reverse Transcriptase
• SuperScript II Reverse Transcriptase
• SuperScript III Reverse Transcriptase

M-MLV Reverse Transcriptase Buffer does NOT contain actinomycin D, nucleotides, or dithiothreitol (DTT).

Order Info

Approved for shipment on wet or dry ice

Specifications

Concentration 5X
For Use With (Application) Reverse Transcription
Quantity 1 mL
Includes

• M-MLV Reverse Transcriptase Buffer (1 mL)
• 0.1 M DTT (500 μL)

Store at –20°C.

Frequently Asked Questions (FAQs)

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

What is the highest temperature that MMLV, SuperScript II, SuperScript III, or SuperScript IV RTs can be used?

The optimal temperature for for MMLV is 42 degrees C. The optimal temperature for SuperScript II RT is 42 degrees C, and can be used up to 50 degrees C. The optimal temperature for SuperScript III RT is 50 degrees C, and can be used up to 55 degrees C. For some qRT-PCR reactions where gene-specific primers are used, you can do the RT reaction at 60 degrees C. The optimal temperature for SuperScript IV RT is 50 degrees C, but can be used up to 65 degrees C.

Can I use elevated temperatures for reverse transcription with Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase?

Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is not well-suited for elevated temperatures. Typically, M-MLV RT operates optimally at temperatures around 37 degrees C to 42 degrees C.

To perform reverse transcription at elevated temperatures (e.g., 50 degrees C or higher), consider using thermostable reverse transcriptases such as SuperScript IV or Maxima H Minus, which are engineered to perform efficiently at higher temperatures.

For Research Use Only. Not for use in diagnostic procedures.