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Invitrogen™ Drosophila S2 Cells in Schneider's Medium

Catalog No. R69007
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R69007 1 mL
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Catalog No. R69007 Supplier Invitrogen™ Supplier No. R69007
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Description

Derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos

Gibco Drosophila S2 cells are used for heterologous protein expression in the DrosophilaM Expression System (DES™). Many features of the S2 cell line suggest that it is derived from a macrophage-like lineage. S2 cells grow at room temperature in ambient CO2 as a loose, semi-adherent monolayer in tissue culture flasks and in suspension in spinners and shake flasks.

  • Expression of protein transiently or through generation of stable cell lines
  • Quality and performance testing

Cell Culture, Drosophila S2 Cell Culture, Insect Cell Culture, Insect Expression, Insect Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Content And Storage Storage conditions: Liquid nitrogen (vapor phase)
Shipping conditions: Frozen
Cell Line S2 (Schneider 2)
Cell Type Insect Cells
Species Drosophila
Product Type Drosophila S2 Cells
Quantity 1 mL

Frequently Asked Questions (FAQs)

What is the doubling time of Drosophila S2 cells?

When grown at the optimal temperature of 27-28ºC, Drosophila S2 cells grow very quickly, with an approximate 14-16 hr doubling time. The cells can also be grown at 22-25ºC with doubling time of 24hrs. Humidity is an issue, so a shallow pan of water can be left in the incubator, but the incubator does not need to be turned on.

Growth medium is also a factor in doubling time - the doubling times listed here are for Sf900 II medium.

The cells can reach maximum densities of 1 to 2.5 x 10E7 cells/ml in shake flask cultures.
I am working with S2 cells that my lab has purchased from you. Can you please let me know which solvent I should use to dissolve the CuSO4?

CuSO4 is water soluble. You can dissolve it in water for cell culture use, and sterile-filter it.

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.