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Invitrogen™ FreeStyle™ CHO-S Cells

Catalog No. R80007
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Catalog No. R80007 Supplier Invitrogen™ Supplier No. R80007
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Description

Rapid and high-yield mammalian protein production

Animal origin-free, chemically defined, low-protein, reduced growth factors, reduced serum, xeno-free. The FreeStyle MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle cells with high efficiency. The FreeStyle MAX Expression System combines GIBCO™ FreeStyle Media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.

  • FreeStyle MAX System provides:An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
  • Functional proteins (up to milligram quantities) with all post-translational modifications produced in one week
  • Simplified downstream purification of secreted proteins with FreeStyle serum-free medium
  • Straightforward protocol for production scale-up
  • Replace time-consuming stable cell line generation with large-scale functional protein production in one week

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture, Mammalian Expression, Mammalian Expression Cell Lines, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation and Analysis, Transfection, Viral Vaccine Production

Specifications

Cell Line FreeStyle™ CHO-S Cells
Content And Storage 1 vial of 1 x 107 cells. Store in liquid nitrogen.
Cell Type CHO-S
Transfection Technique Lipid-based Transfection
Species Hamster
For Use With (Application) cand Transfection
Product Line Freestyle
Product Type CHO-S Cell
Quantity 1 mL
Starting Material Amount 1 x 107
System Type FreeStyle™
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Frequently Asked Questions (FAQs)

How do FreeStyle 293-F cells compare to FreeStyle CHO-S cells in terms of antibody and protein yield?

When comparing FreeStyle 293-F cells with FreeStyle CHO-S cells for average range of antibody and protein yield, it is definitely expected that antibody yield will be higher in FreeStyle 293-F cells than in FreeStyle CHO-S cells, when generated by transient transfection. On the other hand, antibody yields in stable CHO cells can be much higher. For non-antibody proteins, it is impossible to predict which of the two cell lines may provide higher yields.
How long can I keep culturing FreeStyle cells after transfection?

We typically keep the culture going for 7-10 days posttransfection. After that, protein expression may drop.

For Research Use Only. Not for use in diagnostic procedures.