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Invitrogen™ Electroporation Cuvettes, 0.4 cm

Catalog No. P46050
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50 Cuvettes/Bag
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P46050 50 Cuvettes/Bag
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Catalog No. P46050 Supplier Invitrogen™ Supplier No. P46050
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Description

Electroporation uses short, high-voltage electric pulses to overcome the cell membrane and introduce DNA directly into cells. These cuvettes are specifically designed help achieve high DNA transformation efficiency during the introduction of DNA into bacterial, yeast, fungal, and mammalian cells.

Electroporation uses short, high-voltage electric pulses to overcome the cell membrane and introduce DNA directly into cells. These cuvettes are specifically designed help achieve high DNA transformation efficiency during the introduction of DNA into bacterial, yeast, fungal, and mammalian cells.

Our electroporation cuvettes are precision manufactured from the highest quality materials to ensure optimal and reproducible transformation results.

Electroporation cuvette features
• Compatible with virtually all common electroporation devices
• Gamma-irradiated and individually packaged to ensure sterility
• Color-coded snap cap for easy identification of gap size (red cap for 0.4 cm cuvette)

Note: Electroporation cuvettes are recommended for use with all of our electroporation competent cells.

Specifications

For Use With (Equipment) Electroporation Device
Product Type Electroporation Cuvette
Diameter (Metric) 0.4 cm
Quantity 50 Cuvettes/Bag
Content And Storage • 50 individually wrapped, sterile cuvettes

Store at room temperature.
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Will Invitrogen cuvettes fit my electroporator?

Our cuvettes are known as the "Potter-style" cuvettes, and they fit most electroporators that accept a standard size cuvette.
Are Invitrogen electroporator cuvettes autoclavable?

No, one should not autoclave these cuvettes. They are gamma-irradiated to ensure sterility.
What electroporation conditions are recommended for transfection of EcR-293 cells?

5 µg of DNA for 1 x 10e6 cells in a 0.4 cm cuvette. Electroporator settings are routinely 500 µF and 250 Volts but this may have to be optimized depending upon the make and model of the electroporator. Following electroporation, cells are plated out onto 100 mm dishes and media is changed to selective media (400 µg/ml Zeocin) after 24 hours recovery time.
For mammalian electroporation, do we use carrier DNA? If so, how much and what type?

Carrier DNA is unnecessary for mammalian transfection by electroporation. Invitrogen typically uses 10 µg of target DNA WITHOUT carrier DNA.
Is it possible to electroporate eukaryotic cells in 0.1 cm cuvettes?

With the exception of some fungi, 0.1 cm cuvettes are not used for electroporation of eukaryotic cells. The field strength may be set to an appropriate value but the pulse lengths will typically be too short because of the high conductance of the electroporation medium (due to the short electrode gap distance). The efficiencies of transformation are typically 0.5 - 2 logs lower. Please follow manufacturer's recommended conditions for electroporation.

For Research Use Only. Not for use in diagnostic procedures.