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Invitrogen™ Dynabeads™ Mouse CD43 (Untouched™ B Cells)
Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes.
Supplier: Invitrogen™ 11422D
Includes: 5mL MyOne; Dynabeads- coated with streptavidin and biotinylated rat monoclonal CD43 antibody against mouse T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes
Description
Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated mouse B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.
High purity, recovery and viability of mouse untouched B cells
• Easy to use and to scale up
• No columns required
Methodology
Dynabeads Mouse CD43 (Untouched B Cells) is added directly to the sample and binds the unwanted cells. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched mouse B cells can be directly analyzed in a flow cytometer and used in any application.
Starting Sample
Spleen or lymph nodes
Order Info
Shipping Condition: Room Temperature
Specifications
| B Cells | |
| Antibody Coated Bead | |
| Bead | |
| Processes ∼2 x 109 cells/kit, Processes ∼2 x 109 cells total, Processes ∼1 x 109 cells/kit | |
| DYNAL, Dynabeads, Untouched | |
| 1 kit | |
| Lymph Node, Tissue Digests, Lymph nodes, Spleen | |
| 5 x 107 cells/test |
| The Kit Contains: 5 mL MyOne™ Dynabeads™-coated with streptavidin and biotinylated rat monoclonal CD43 antibodyagainst mouse T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. Store at 2°C to 8°C. |
|
| Cells | |
| Negative Isolation | |
| 40 | |
| Research Grade | |
| Mouse | |
| Room Temperature | |
| Mouse |
For Research Use Only. Not for use in diagnostic procedures.