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Invitrogen™ Dynabeads™ Mouse CD43 (Untouched™ B Cells)

Catalog No. 11422D
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Catalog No. 11422D Supplier Invitrogen™ Supplier No. 11422D
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Includes

5mL MyOne; Dynabeads- coated with streptavidin and biotinylated rat monoclonal CD43 antibody against mouse T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes

Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes.

Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated mouse B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

High purity, recovery and viability of mouse untouched B cells
• Easy to use and to scale up
• No columns required

Methodology
Dynabeads Mouse CD43 (Untouched B Cells) is added directly to the sample and binds the unwanted cells. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched mouse B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample
Spleen or lymph nodes

Order Info

Shipping Condition: Room Temperature

Specifications

Cell Type B Cells
Content And Storage The Kit Contains: 5 mL MyOne™ Dynabeads™-coated with streptavidin and biotinylated rat monoclonal CD43 antibodyagainst mouse T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes.

Store at 2°C to 8°C.
Product Type Antibody Coated Bead
Final Product Type Cells
Format Bead
Isolation Technology Negative Isolation
No. of Cells Processes ∼2 x 109 cells/kit, Processes ∼2 x 109 cells total, Processes ∼1 x 109 cells/kit
No. of Tests 40
Product Line DYNAL, Dynabeads, Untouched
Purity or Quality Grade Research Grade
Quantity 1 kit
Reactivity Mouse
Sample Type Lymph Node, Tissue Digests, Lymph nodes, Spleen
Shipping Condition Room Temperature
Starting Material Cell No. 5 x 107 cells/test
Target Species Mouse
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For Research Use Only. Not for use in diagnostic procedures.