missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. dNTPs
  6. Invitrogen™ dNTP Mix (10 mM ea)

Invitrogen™ dNTP Mix (10 mM ea)

Catalog No. 18427089
Change view
Click to view available options
Quantity:
100 μL
1 mL
5 x 1 mL
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
18427089 5 x 1 mL
18427088 1 mL
18427013 100 μL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Catalog No. 18427089 Supplier Invitrogen™ Supplier No. 18427089
Only null left

Description

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes.

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes including: polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Features include:
• Chemically synthesized
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS)

Thermo Fisher Scientific offers a wide variety of dNTPs in different concentrations, volumes, and formats.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Concentration 10 mM
Content And Storage dNTP Mix, contains 10 mM each of dATP, dCTP, dGTP, and dTTP (1 x 1 mL)

Store at –20°C.
Form Liquid
Label or Dye Unlabeled
Quantity 5 x 1 mL
Shipping Condition Approved for shipment on Wet or Dry Ice
For Use With (Application) Standard PCR, RT-PCR, Real Time PCR (qPCR)

Frequently Asked Questions (FAQs)

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

Why do some dNTP mixes contain dTTP and others TTP?

Thymine is normally found as a deoxyribose. Thus, TTP and dTTP are essentially the same thing. Both types of mixes can be used in PCR reactions
What concentration of dNTPs is typically used in a PCR amplification?

The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.

For Research Use Only. Not for use in diagnostic procedures.