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Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment

Catalog No. 18012021
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18012021 100 Units
18012039 500 Units
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Catalog No. 18012021 Supplier Invitrogen™ Supplier No. 18012021
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Description

Includes

DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500mM Tris-HCl (pH 8.0), 100mM MgCl2, 500mM NaCl], vial of dilution buffer.

DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities

Applications:
Fill-in of 5' overhangs . Synthesis of probes by random primers labeling method. Sequencing single- and double-stranded DNA. Site-directed mutagenesis.

Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition:
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.

Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

Content And Storage DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM
MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C.
Description DNA Polymerase
Polymerase DNA Polymerase I
Exonuclease Activity 3' to 5'
Quantity 100 Units
Shipping Condition Approved for shipment on Wet or Dry Ice
Hot Start No

For Research Use Only. Not for use in diagnostic procedures.