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Invitrogen™ ChargeSwitch™ Plasmid ER Mini Kit
ChargeSwitch™ Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA.
Supplier: Invitrogen™ CS10100
Includes:
- ChargeSwitch Magnetic Beads (25 mg/mL) in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20
- RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
- Resuspension Buffer (R4; 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
- ChargeSwitch Lysis Buffer (L9)
- ChargeSwitch Precipitation Buffer (N5)
- ChargeSwitch Wash Buffer (W11)
- ChargeSwitch Wash Buffer (W12)
- ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
- Endotoxin Reduction Reagent (D1; ETRR)
Requires : Magnetic rack
Description
ChargeSwitch™ Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA from 1-5 mL of fresh, overnight bacterial cultures without the use of chaotropic salts, organic solvents, or ethanol, to yield up to 20 μg of transfection-grade plasmid DNA in a total time of about 10 minutes after sample preparation.
- Optimized for higher-purity DNA extraction
- Improved performance with accompanying magnetic rack
- Cleaner purified plasmid for optimal downstream performance
- High quality, high yield plasmid DNA
- Compatible with MagnaRack™ magnetic rack
- Reliable performance in variety of downstream applications
Order Info
Shipping Condition: Room Temperature
Specifications
All components of the ChargeSwitch™ Plasmid ER (Endotoxin Reduced) Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Store Precipitation Buffer (N5) at 4°C. Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store the remaining components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
|
| ChargeSwitch Chemistry | |
| Bacterial Culture | |
| Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription | |
| < 100 μg (Small-Scale) Plasmid DNA | |
| Plasmid ER Mini Kit | |
| Room Temperature | |
| 10 min. |
| Kit | |
| Mini | |
| Plasmid DNA | |
| Not High-throughput Compatible (Manual) | |
| ChargeSwitch | |
| 50 Preps | |
| Plasmid DNA |
For Research Use Only. Not for use in diagnostic procedures.