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  5. Invitrogen™ ChargeSwitch™ gDNA Buccal Cell Kit

Invitrogen™ ChargeSwitch™ gDNA Buccal Cell Kit

Catalog No. CS11021
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CS1102110 960 Preps
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Catalog No. CS11021 Supplier Invitrogen™ Supplier No. CS11021
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Description

Includes

ChargeSwitch Lysis Buffer (L11)
ChargeSwitch Magnetic Beads
Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
ChargeSwitch Purification Buffer (N6)
ChargeSwitch Wash Buffer (W12)
ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

Requires

Use of a magnetic accessory.

ChargeSwitch gDNA Buccal Cell Kit maximizes the yield of high quality genomic DNA from buccal samples for rapid buccal swab processing.

  • Sample size 1 buccal swab; Typical performance up to 6 μg
  • Maximum yield sensitivity
  • Rapid single-tube protocol, suitable for automation
  • High-quality DNA for downstream Single Tandem Repeat (STR) analysis
  • ChargeSwitch gDNA Buccal Cell Kit maximizes the yield of high quality genomic DNA from buccal samples for rapid buccal swab processing

Order Info

Shipping Condition: Room Temperature

Specifications

Cell Type Buccal Cell
Content And Storage

All components of the ChargeSwitch™ gDNA Buccal Cell Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.

Components:
• ChargeSwitch™ Lysis Buffer (L11)
• ChargeSwitch™ Magnetic Beads
• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
• ChargeSwitch™ Purification Buffer (N6)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format Kit
Isolation Technology ChargeSwitch Chemistry
Sample Type Buccal Samples
For Use With (Application) Forensic
High-throughput Compatibility High-throughput Compatible
No. of Reactions 50 Preps
Product Line ChargeSwitch
Product Type gDNA Buccal Cell Kit
Quantity 50 Preps
Shipping Condition Room Temperature
Target Genomic DNA
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Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.
What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

I want to isolate genomic DNA from buccal cells. How do I do this?

We would recommend using one of our MagMAX DNA Multi-Sample Ultra 2.0 Kit or ChargeSwitch gDNA Buccal Cell Kits, which were designed to isolate high-quality genomic DNA from buccal samples. We also offer normalized kits, which will normalize isolated DNA concentration from sample to sample.
How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.