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Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity DFS Item

Amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and a proofreading enzyme, and AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases.

$754.00 - $2,910.00

Specifications

Storage Requirements Store at -30° to -10°C
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: AccuPrime™ Taq DNA Polymerase High Fidelity (40μL, at 5U/μL)) is supplied with: 50mM MgSO4 (1mL), 10X AccuPrime™ PCR Buffer I (1mL), 10X AccuPrime™ PCR Buffer II (1mL)

Buffer I and II differ in their concentration of thermostable AccuPrime™ protein. Their components are: 600mM Tris-SO4 (pH 8.9), 180mM (NH4)2SO4, 20mM MgSO4, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol. The supplied PCR Buffer is a 10X concentrate and should be diluted for use. Buffer I is optimized for plasmids, cDNA, and λDNA. Buffer II is optimized for genomic DNA.


Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
12346086 Life Technologies
LS12346086
200 reactions Each for $754.00
12346094 Life Technologies
LS12346094
1000 reactions Each for $2,910.00
Specifications

Description

  • High specificity and yield for the most robust PCR amplification
  • 9-fold higher fidelity than TaqDNA polymerase alone
  • Mimimal optimization steps, even with nonoptimized primer sets
  • Efficient amplification of targets over a broad size range up to 20kb
How it work
High fidelity is achieved by a combination of Platinum antiTaqDNA polymerase antibodies that inhibit polymerase activity, providing an automatic “hot-start”, and the proofreading (3'-5' exonuclease activity) enzyme Pyrococcusspecies GB-D. The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Long Fragment PCR, Methylation Analysis, PCR, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research

Order Info

Shipping Condition: Dry ice

Description & Specifications

Specifications

Store at -30° to -10°C