Please login to your online account to display your discounted pricing

Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity DFS Item

Amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and a proofreading enzyme, and AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases.

$730.80 - $2,866.50

Specifications

Storage Requirements Store at -30° to -10°C
View More Specs
Includes:  AccuPrime™ Taq DNA Polymerase High Fidelity (40μL, at 5U/μL)) is supplied with: 50mM MgSO4 (1mL), 10X AccuPrime™ PCR Buffer I (1mL), 10X AccuPrime™ PCR Buffer II (1mL)

Buffer I and II differ in their concentration of thermostable AccuPrime™ protein. Their components are: 600mM Tris-SO4 (pH 8.9), 180mM (NH4)2SO4, 20mM MgSO4, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol. The supplied PCR Buffer is a 10X concentrate and should be diluted for use. Buffer I is optimized for plasmids, cDNA, and λDNA. Buffer II is optimized for genomic DNA.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

12346086

DFS Item
 
life technologies
12346086
200 reactions Each for $730.80

12346094

DFS Item
 
life technologies
12346094
1000 reactions Each for $2,866.50
Description & Specifications

Specifications

Storage Requirements Store at -30° to -10°C

  • High specificity and yield for the most robust PCR amplification
  • 9-fold higher fidelity than TaqDNA polymerase alone
  • Mimimal optimization steps, even with nonoptimized primer sets
  • Efficient amplification of targets over a broad size range up to 20kb
How it work
High fidelity is achieved by a combination of Platinum antiTaqDNA polymerase antibodies that inhibit polymerase activity, providing an automatic “hot-start”, and the proofreading (3'-5' exonuclease activity) enzyme Pyrococcusspecies GB-D. The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Long Fragment PCR, Methylation Analysis, PCR, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research

Shipping Condition: Dry ice