Please login to your online account to display your discounted pricing

Invitrogen™ AccuPrime™ Pfx DNA Polymerase DFS Item

Provides higher fidelity than Pfu DNA polymerase

$564.90 - $2,541.00

Specifications

Fidelity (vs. Taq) Fidelity (vs. Taq): 26X
Gel Compatibility Gel Compatibility: Low
Storage Requirements Store at -20°C.
View More Specs
Includes:  AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

12344032

DFS Item
 
life technologies
12344032
1000 reactions Each for $2,541.00

12344024

DFS Item
 
life technologies
12344024
200 reactions Each for $564.90
Description & Specifications

Specifications

Fidelity (vs. Taq) Fidelity (vs. Taq): 26X
Gel Compatibility Gel Compatibility: Low
Storage Requirements Store at -20°C.

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.

  • Achieve greater than 26 times higher fidelity than Taq DNA polymerase
  • Amplify fragments up to 12 kb
  • Minimize PCR optimization
  • Built-in hot start

Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

Approved for shipment on Wet or Dry Ice