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Invitrogen™ AccuPrime™ Pfx DNA Polymerase DFS Item

AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.

$764.00 - $4120.00

Specifications

Detection Method Primer-probe
GC-Rich PCR Performance Low
Polymerase AccuPrime Pfx DNA Polymerase
Reaction Speed Standard
Shipping Condition Wet or Dry Ice
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Includes: AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.

Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity  
Catalog Number Mfr. No. No. of Reactions Price Quantity  
12344024 DFS Item
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Invitrogen™
12344024
200 Reactions
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12344032 DFS Item
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Invitrogen™
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Description

Description

AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum™ anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum™ Pfx alone.

With AccuPrime™ Pfx DNA Polymerase you will:
• Achieve greater than 26 times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimization

Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime™ Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Specifications

Primer-probe
AccuPrime Pfx DNA Polymerase
Wet or Dry Ice
26X
Blunt
12 kb or less
Low
Standard
Hot-start PCR, High-fidelity PCR
Built-In Hot Start
Separate Components
SDS
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For Research Use Only. Not for use in diagnostic procedures.