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Invitrogen™ AccuPrime™ Pfx DNA Polymerase 
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
Supplier: Invitrogen™ 12344024
Description
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.
Benefits of AccuPrime Pfx DNA Polymerase
- Greater than 26 times higher fidelity than Taq DNA polymerase
- Amplification of fragments up to 12 kb
- Minimized PCR optimization
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- High-fidelity PCR
- Hot-start PCR
Notes
- AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
- The 10X AccuPrime Pfx reaction mix contains MgSO4, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.
Greener Choice Claims
- Environmental benefits include:
- Renewable Energy
Specifications
| • AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL) • 10X AccuPrime Pfx Reaction Mix (1 x 1 mL) • 50 mM MgSO4 (1 x 1 mL) Store at -20°C. |
|
| Low | |
| Standard | |
| Wet or Dry Ice | |
| 26X | |
| 200 Reactions | |
| Separate Components | |
| 100 ÎĽL |
| Primer-probe | |
| AccuPrime Pfx DNA Polymerase | |
| 200 Reactions | |
| Hot-start PCR, High-fidelity PCR | |
| Built-In Hot Start | |
| Blunt | |
| 12 kb or less |
For Research Use Only. Not for use in diagnostic procedures.