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Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase DFS Item

High-specificity, high-yield PCR amplification of GC-rich templates

$488.25 - $2,152.50

Specifications

Fidelity (vs. Taq) Fidelity (vs. Taq): 2X
Gel Compatibility Gel Compatibility: High
Storage Requirements Store at -20°C.
View More Specs
Includes:  AccuPrime GC-Rich DNA Polymerase (100μL, at 2U/μL) is supplied with two 5X reaction buffers and a separate vial (1mL) of 50mM MgSO4 . Both buffers contain dNTPs, MgSO4 , and AccuPrime accessory proteins. Reaction buffer A (1mL) is for genomic targets. Reaction buffer B (1mL) is for use with cDNA, plasmid, or lambda targets. Sufficient reagents are provided for 200 amplification reactions of 25μL each (at 1 unit of enzyme per reaction).

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

12337024

DFS Item
 
life technologies
12337024
1000 reactions Each for $2,152.50

12337016

DFS Item
 
life technologies
12337016
200 reactions Each for $488.25
Description & Specifications

Specifications

Fidelity (vs. Taq) Fidelity (vs. Taq): 2X
Gel Compatibility Gel Compatibility: High
Storage Requirements Store at -20°C.

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).

  • Yield: high yields for targets up to 5kb in length
  • Specificity: AccuPrime accessory proteins for improved PCR specificity
  • Sensitivity: sensitivity down to 5ng of template DNA
  • Robust and specific amplifications
  • Built-in hot start

The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition
One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10nmoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.

Amplification of Bisulfite-Treated DNA, GC-Rich PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

Approved for shipment on Wet or Dry Ice