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  7. Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

Catalog No. 12337016
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12337016 200 Reactions
12337024 1000 Reactions
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Catalog No. 12337016 Supplier Invitrogen™ Supplier No. 12337016
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Description

Includes

AccuPrime GC-Rich DNA Polymerase (100μL, at 2U/μL) is supplied with two 5X reaction buffers and a separate vial (1mL) of 50mM MgSO4 . Both buffers contain dNTPs, MgSO4 , and AccuPrime accessory proteins. Reaction buffer A (1mL) is for genomic targets. Reaction buffer B (1mL) is for use with cDNA, plasmid, or lambda targets. Sufficient reagents are provided for 200 amplification reactions of 25μL each (at 1 unit of enzyme per reaction).

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).

AccuPrime GC-Rich DNA Polymerase features

  • High yields for targets up to 5 kb in length
  • AccuPrime accessory proteins for improved PCR specificity
  • Sensitivity down to 5 ng of template DNA

Robust and specific amplifications

The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition

One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min. at 74°C.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Content And Storage • AccuPrime GC-Rich DNA Polymerase (1 x 100 μL at 2 U/μL)
• 5X AccuPrime GC-Rich Buffer A (1 x 1 mL)
• 5X AccuPrime GC-Rich Buffer B (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)

Store at –20°C.
GC-Rich PCR Performance High
Polymerase AccuPrime GC-Rich DNA Polymerase
Reaction Speed Standard
Quantity 200 rxns
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR
Fidelity (vs. Taq) 2X
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Overhang Blunt
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Volume 100 μL
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For Research Use Only. Not for use in diagnostic procedures.