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Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
Supplier: Invitrogen™ 12337016
Includes: AccuPrime GC-Rich DNA Polymerase (100μL, at 2U/μL) is supplied with two 5X reaction buffers and a separate vial (1mL) of 50mM MgSO4 . Both buffers contain dNTPs, MgSO4 , and AccuPrime accessory proteins. Reaction buffer A (1mL) is for genomic targets. Reaction buffer B (1mL) is for use with cDNA, plasmid, or lambda targets. Sufficient reagents are provided for 200 amplification reactions of 25μL each (at 1 unit of enzyme per reaction).
Description
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).
AccuPrime GC-Rich DNA Polymerase features
- High yields for targets up to 5 kb in length
- AccuPrime accessory proteins for improved PCR specificity
- Sensitivity down to 5 ng of template DNA
Robust and specific amplifications
The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
Unit definition
One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min. at 74°C.
Order Info
Approved for shipment on Wet or Dry Ice
Chemical Identifiers
| High | |
| Standard | |
| Hot-start PCR | |
| Built-In Hot Start | |
| Separate Components |
| AccuPrime GC-Rich DNA Polymerase | |
| Wet or Dry Ice | |
| 2X | |
| Blunt | |
| 5 kb or less |
Specifications
| • AccuPrime GC-Rich DNA Polymerase (1 x 100 μL at 2 U/μL) • 5X AccuPrime GC-Rich Buffer A (1 x 1 mL) • 5X AccuPrime GC-Rich Buffer B (1 x 1 mL) • 50 mM MgSO4 (1 x 1 mL) Store at –20°C. |
|
| AccuPrime GC-Rich DNA Polymerase | |
| 200 rxns | |
| Hot-start PCR | |
| Built-In Hot Start | |
| Blunt | |
| 5 kb or less |
| High | |
| Standard | |
| Wet or Dry Ice | |
| 2X | |
| 200 Reactions | |
| Separate Components | |
| 100 μL |
For Research Use Only. Not for use in diagnostic procedures.