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Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A)

Catalog No. 501128915
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501128915 1 kit
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Contains Sodium azide and formaldehyde
Catalog No. 501128915 Supplier Invitrogen™ Supplier No. 88882388
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Contains Sodium azide and formaldehyde

Description

Includes

  • Brefeldin A solution
  • IC Fixation Buffer
  • Permeabilization Buffer (10X) . Permeabilization Buffer is supplied as a 10X concentrate. 1X Permeabilization Buffer contains 0.1% saponin and 0.09% sodium azide.

Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.

  • Fixation buffer is used to cross-link, stabilize, and fix the cell membrane
  • Permeabilization buffer is used to reversibly permeabilize the cell membrane so the staining antibodies can enter the cell effectively

Specifications

Quantity 1 kit
Reagent Type Fixation Reagent, Permeabilization Reagent
Product Line eBioscience
Product Type Fixation/Permeabilization Buffer

For Research Use Only. Not for use in diagnostic procedures.