Thermo Scientific™ Imject™ Maleimide-Activated mcKLH and Kit
Perform hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens with this sulfhydryl-reactive mariculture KLH.
Manufacturer: Thermo Scientific™ PI77164
Thermo Scientific™ Imject Maleimide-Activated mcKLH is sulfhydryl-reactive immunogenic mariculture keyhole limpet hemocyanin (KLH) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.The high-quality mariculture KLH is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Numerous antigen molecules can be conjugated to each KLH molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Select from among several package sizes of activated KLH protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns.
Convenient packages – choose stabilized, lyophilized Maleimide-Activated mcKLH or a complete immunogen conjugation kit
Optimized activation – specifications require that each lot is activated with more than 400 moles of sulhydryl-reactive maleimide per mole of KLH, ensuring consistently high levels of conjugation
Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production
Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals
High-quality carrier protein – KLH is isolated from the mollusk Megathura crenulata (keyhole limpet); mcKLH is a high-quality form of KLH that is harvested from limpets grown in mariculture
Highly immunogenic – KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390kDa subunits) and elicits a stronger immune response than BSA or ovalbumin
Excellent solubility – our preparations of mariculture KLH are more soluble than typical forms and we also offer a PEGylated form for even greater hydrophilicity and solubility
Kit contains stabilized, lyophilized Maleimide-Activated mcKLH (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization
|Imject Maleimide Conjugation Buffer|
|Maleimide Conjugation Buffer; 30mL|
|0.083M sodium phosphate, 0.1M EDTA, 0.9M NaCl, 0.1M sorbitol and 0.02% sodium azide; pH 7.2|
|Dissolving up to 60mg of sulfhydryl-peptide for maleimide conjugation with carrier proteins|