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Cytiva TempliPhi™ 100/500 Amplification Kit
Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
$471.84 - $2,106.00
Specifications
| Content And Storage | The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0â4°C during handling. DO NOT warm above 4°C prior to amplification |
|---|---|
| Description | illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA. |
| For Use With (Application) | Sequencing Template Amplification Kits and Reagents |
| Includes | 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA |
| Test Time | 4-18 h depending on starting material |
Includes: Sample buffer, Reaction buffer, Enzyme mix, and Positive control pUC19 DNA (2ng/μL)
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
|
45-002-262
|
Cytiva
25640010 |
100 |
N/A
|
|
|||||
|
45-002-265
|
Cytiva
25640050 |
500 |
N/A
|
|
|||||
Description
- Generate microgram quantities of template DNA from picogram amounts of starting material
- Completed reactions contain uniform quantities of DNA irrespective of input amount, allowing for use directly in sequencing reactions without quantification
- Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA without the need for purification
- Simple protocol reduces time, labor, and consumables needed for template preparation, and workflow enables easy automation
- Contain separate, unmixed components for enhanced stability
Specifications
| The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0â4°C during handling. DO NOT warm above 4°C prior to amplification | |
| Sequencing Template Amplification Kits and Reagents | |
| 4-18 h depending on starting material |
| illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA. | |
| 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA |